RNA Extraction
Instructions
- 1ml TRIzol per well. 5 min at RT.
- Add 200µl chloroform, shake 15 sec, incubate 3 min at RT.
- Cfg 15 min at ≤12000 g, 4C.
- Aqueous phase + 0.5ml isopropanol, incubate 10 min at RT.
- Cfg 10 min at ≤12000 g, 4C.
- Wash with 1 ml 75% EtOH, 5 min at ≤7500 g, 4C.
- Dissolve the pellet in µl DEPC H2O.
- Spec 1µl RNA + 99µl H2O (dilution 1:100).